human hcc cell lines snu 387  (ATCC)

Journal: iScience

Article Title: Targeting Smyd3 by next-generation antisense oligonucleotides suppresses liver tumor growth

doi: 10.1016/j.isci.2021.102473

Figure Lengend Snippet: Smyd3-ASO treatment inhibits proliferation rates and colony formation ability of human hepatocellular carcinoma cell lines (A) Growth curves of HuH7, SNU387, HLF, SNU398, SNU423, and SNU449 cell lines treated with 0.2 μM control-ASO (blue lines) or hSmyd3-ASO-1 (red lines). The cells were pretreated with the ASOs for one passage before plating. Data points represent mean values of the fold increase in cell numbers compared to time 0 (12 hr after seeding) and SEM from 3 independent experiments. (B) Colony formation capacity of HLF, SNU398, SNU423, SNU449, and HuH7 cell lines treated with 0.2 μM control-ASO or hSmyd3-ASO-1 or hSmyd3-ASO-2. The cells were seeded at a density of 1000 cells per 100 mm plate and fixed after 18-22 days. ASO-containing medium was replaced every 3 days. Bars represent the average number of colonies per plate and SEM from 3 experiments. See also .

Article Snippet: The human HCC cell lines SNU387 (from grade IV/V pleomorphic HCC), SNU398, SNU423 (from grade III/IV pleomorphic HCC) and SNU449 (from grade II-IV HCC) were from ATCC, HuH7 and HLF cells were from Japanese Cancer Research Foundation (JCRF).

Techniques: Control

Journal: iScience

Article Title: Targeting Smyd3 by next-generation antisense oligonucleotides suppresses liver tumor growth

doi: 10.1016/j.isci.2021.102473

Figure Lengend Snippet:

Article Snippet: The human HCC cell lines SNU387 (from grade IV/V pleomorphic HCC), SNU398, SNU423 (from grade III/IV pleomorphic HCC) and SNU449 (from grade II-IV HCC) were from ATCC, HuH7 and HLF cells were from Japanese Cancer Research Foundation (JCRF).

Techniques: Recombinant, Protease Inhibitor, Reverse Transcription, SYBR Green Assay, Modification, Software

Journal: Oncology Letters

Article Title: MicroRNA-9 enhances sensitivity to cetuximab in epithelial phenotype hepatocellular carcinoma cells through regulation of the eukaryotic translation initiation factor 5A-2

doi: 10.3892/ol.2017.7399

Figure Lengend Snippet: Overexpression of miR-9 enhances sensitivity of epithelial HCC cells to cetuximab. (A-D) Viability of HCC cells treated with cetuximab in the presence of a miR-9 mimic or inhibitor. (E-H) Photomicrographs and bar charts depicting EdU staining and relative EdU-positive ratios, respectively, of HCC cells following treatment with cetuximab, with cetuximab plus a miR-9 mimic or with cetuximab plus miR-9 inhibitor for 48 h. *P<0.05, **P<0.01 vs. control, Hep3B: P miR-9 mimic+Cet =0.0025, P miR-9 inhibitor+Cet =0.0295, Huh7: P miR-9 mimic+Cet =0.0356, P miR-9 inhibitor+Cet =0.0480. All experiments were performed ≥3 times. miR-9, microRNA-9; HCC, hepatocellular carcinoma; EdU, 5-ethynyl-2′-deoxyuridine; Cet, cetuximab.

Article Snippet: Human HCC cell lines (Hep3B, Huh7, SNU387 and SNU449) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Over Expression, Staining, Control

Journal: Oncology Letters

Article Title: MicroRNA-9 enhances sensitivity to cetuximab in epithelial phenotype hepatocellular carcinoma cells through regulation of the eukaryotic translation initiation factor 5A-2

doi: 10.3892/ol.2017.7399

Figure Lengend Snippet: miR-9 modulates expression of eIF-5A-2 in HCC cell lines. (A) miR-9 target site in eIF-5A-2 predicted by TargetScan. (B) Expression of miR-9 and eIF-5A-2 mRNA in HCC cells as determined by RT-qPCR. *P<0.05, **P<0.01, ***P<0.001 vs. Huh7; # P<0.05, ## P<0.01 vs. Hep3B. (C) eIF-5A-2 expression in HCC cells following treatment with a miR-9 mimic or inhibitor as determined by RT-qPCR. **P<0.01, ***P<0.001 vs. the control. (D) Expression levels of miR-9 following treatment with a miR-9 mimic or inhibitor as determined by RT-qPCR. *P<0.05, **P<0.01, ***P<0.001 vs. the control (E) Western blot analysis was used to detect eIF-5A-2 expression in the various HCC cell lines in the presence of the miR-9 mimic, inhibitor and control. *P<0.05 vs. negative siRNA. miR-9, microRNA-9; eIF-5A-2; eukaryotic translation initiation factor 5A-2; HCC, hepatocellular carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; miRNA, microRNA; UTR, untranslated region.

Article Snippet: Human HCC cell lines (Hep3B, Huh7, SNU387 and SNU449) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: MicroRNA-9 enhances sensitivity to cetuximab in epithelial phenotype hepatocellular carcinoma cells through regulation of the eukaryotic translation initiation factor 5A-2

doi: 10.3892/ol.2017.7399

Figure Lengend Snippet: Sensitivity of HCC cell lines to cetuximab. (A) Western blot analysis of eIF-5A-2 expression in eIF-5A-2 siRNA-transfected cells. GAPDH served as a loading control. Knockdown of eIF-5A-2 enhanced the sensitivity of the epithelial phenotype HCC cells, (B) Hep3B and (C) Huh7, to cetuximab, but had no significant effect on the mesenchymal phenotype HCC cells, (D) SNU387 and (E) SNU449. *P<0.05 vs. negative siRNA. HCC, hepatocellular carcinoma; eIF-5A-2; eukaryotic translation initiation factor 5A-2; siRNA, small interfering RNA.

Article Snippet: Human HCC cell lines (Hep3B, Huh7, SNU387 and SNU449) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Western Blot, Expressing, Transfection, Control, Knockdown, Small Interfering RNA